Regenerasi Tunas Adventif Dari Eksplan Daun Tembesu (Fagraea Fragrans Roxb.) Melalui Teknik Kultur Jaringan (Regeneration of Adventitious Shoots From Leaf Explant of Tembesu (Fagraea Fragrans Roxb.) by Tissue Culture)

摘要

Tembesu (Fagraea fragrans Roxb.) is one of native tree species in peatland forest and has high economical values. Sufficient amount in time of qualified seedlings is needed to support peatland rehabilitation program, and it can be achieved by tissue culture. The objective of the research was to find out the best modification medium of MS based on concentration of nitrogen and BAP for induction and multiplication of adventitious shoot from leaves. The protocol of tissue culture consisted of preparation of plant material, adventitious shoots induction, shoots multiplication, shoots elongation, rooting and seedling acclimatization. The results showed (1) addition of BAP 1,5 ppm on MS medium (80 mmol N) induced adventitious shoots from leaves; (2) addition of BAP 0,1 ppm on MS medium (80 mmol N) stimulated the highest multiplication of shoots; (3). clone 2 was the best explant on elongation and rooting stage; (4). clone 4 was the best explant in acclimatization stage.